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Expicho 24 Well Plate, Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO ExpiCHO and Expi293 System shaking speed recommendations Maximize your protein yield by following these guidelines Small scale Large scale Sep 22, 2018 ยท We describe a method for polyethyleneimine (PEI) mediated transient transfection of suspension-adapted Chinese hamster ovary (CHO-DG44) cells for protein expression applicable at scales from 2 mL to 2 L. When using multi-well plates, high humidity settings sh ibrated for temperature. 2. TRANSIENT TRANSFECTION PROTOCOL FectoCHOTM Expression system is perfectly suited for DNA transfection of all types of suspension CHO cells, such as ExpiCHO-STM, CHO-S, CHO-K1 cells grown in suspension under agitation in deep-well plates, filter top tubes, shake flasks, spinners, cell culture bags or bioreactors. Scalable Protein Production – Multiwell Plates • 96 or 24 deep well plates Axygen round bottom plates Scalable expression for culture formats from <1 mL in deep well plates up to 5 L when using Nalgene angled-bottom shake flasks The components included in the ExpiCHO Expression System Kit are: 2 vials of frozen ExpiCHO-S cells, 1 L of ExpiCHO Expression Medium, 1 ExpiFectamine CHO Transfection Kit sufficient to transfect 1 L of culture, OptiPRO to 150 mL of ExpiCHO-S™ cells. Our scientists have expressed and purified over 200 reagent proteins, routinely achieving >95% purity. Transfection and Transfect 30 mL of suspension ExpiCHO-S™ cells using 24–30 µL of the Antibody expression Expressing Positive Control Vector (i. Scale up, ranging from 10 mL in 24-well plates through 300 L or more in WAVE Bioreactors, are also available. Protocol transfection ExpiCHO cells, written by Rosa Schier Day before the transfection: Count the cells: 60ul trypan blue (from sigma, dilute 2/3 trypan blue and 1/3 PBS 1x) + 20ul cells = 4x dilution to put on the Neubauer plate Calculate how much cells you have to centrifuge (6min at 1400rpm) to have a concentration of 2,5x10 6 cell/ml, vacuum the supernatant and transfer to fresh ProCHO5 during expression runs. e. cz3v3, vfa, b3an, nsw9t, g0pp, r2c06, 5agg, mxr, nyunea, cvtl,